Simulation of long-term nutrient removal in a full-scale closed-loop bioreactor for sewage treatment: an example of Bayesian inference

In this study, the performance of nitrogen and phosphorus removal in a full-scale closed-loop bioreactor (oxidation ditch) system was simulated using the ASM2d model. Routine data describing the process for two years were compiled for calibration and validation. To overcome the identifiability problem, the classic Bayesian inference approach was utilized for parameter estimation. The calibrated model could describe the long-term trend of nutrient removal and short-term variations of the process performance, showing that the Bayesian method was a reliable and useful tool for the parameter estimation of the activated sludge models. The anoxic phosphate uptake by polyphosphate accumulating organisms (PAO) contributed 71.2% of the total Poly-P storage, which reveals the dominance of denitrifying phosphorus removal process under the oxygen limiting conditions. It was found that 58.7% of the anoxic Poly-P storage and denitrification by PAO in the reactor was achieved in the aerated compartment, implying that the PAO’s anoxic activity was significantly stimulated by the low dissolved oxygen (DO) level in this compartment due to the oxygen gradient caused by brush aerator.     

Lichens as a useful mapping tool?—an approach to assess atmospheric N loads in Germany by total N content and stable isotope signature

To assess whether nitrogen (N) content and δ¹⁵N ratios in nitrophytic lichen species (Xanthoria parietina (L.) Th. Fr. (1860) and Physcia spp. (Schreb.) Michaux (1803)) reflect the quantity and quality of atmospheric N loads, 348 lichen samples from 174 sampling grid cells were investigated in the western part of Germany. The analysed lichen N content ranged between 0.98 and 4.28 % and δ¹⁵N ratios between ?15.2 and ?1.3?‰. Based on the N concentrations and the δ¹⁵N ratios of lichens, different landscape categories and coupled N deposition rates could be inferred for different regions of Germany. By analysing environmental variables like altitude, ammonia emission density, livestock unit and different defined deposition types, a direct relationship was found between lichen chemistry and N compounds produced from agricultural activity. The results support the development of a monitoring method which could be used nationally or even internationally to support current N deposition measurements, by providing reliable information on the quantity and quality of N deposition in high N environments.     

Biocatalytic dechlorination of hexachlorocyclohexane by immobilized bio-Pd in a pilot scale fluidized bed reactor

Lindane (??-hexachlorocyclohexane, ??-HCH) is a recalcitrant and toxic organochlorine insecticide. Due to its non-selective production process and widespread use, HCH isomers and their degradation products have been detected frequently in soils and groundwater. An innovative technology using microbial produced Pd(0) nanoparticles, i.e. bio-Pd, was developed to treat groundwater containing a mixture of HCHs and chlorobenzenes. In a first step, the groundwater was de-ironized and most of the chlorobenzenes were removed in a biological trickling filter. The ??g?L^?1 levels of HCHs and chlorobenzenes were removed in a second step by the bio-Pd-based technology. Therefore, a 200-L pilot scale reactor was developed with 100?mg?L^?1 bio-Pd encapsulated in alginate beads. Hydrogen gas was bubbled at the bottom of the reactor and served to charge the bio-Pd catalyst. The reactor influent contained 5.2???g?L^?1 HCHs and 51.1???g?L^?1 chlorobenzenes. During a test period of 10?days, 29% of the HCH isomers and 63% of the chlorobenzenes were removed applying a nominal hydraulic residence time of 4?h. These removal percentages could be increased to 75 and 68% by doubling the nominal hydraulic residence time to 8?h. This study demonstrated that biologically produced nanoparticles of Pd can be applied for the large-scale remediation of groundwater contaminated with HCHs.     

The relation between the estimated dietary intake of PCDD/Fs and levels in blood in a Flemish population (50-65 years)

Dioxin-like activity was measured in the serum of 1425 Flemish men and women via the CALUX assay. The adults, aged between 50 and 65 years, participated in a large biomonitoring program, executed by the Flemish Center of Expertise for Environment and Health between 2002 and 2006. Within the context of this biomonitoring program also dietary intake of dioxin-like contaminants was assessed through a food frequency questionnaire. The relation between the estimated dietary intake and the dioxin-like activity in serum was evaluated using multivariate analyses: a logistic model was performed on the total population, while a linear regression analysis was done on the subsample with quantifiable dioxin activity levels in serum. Region, gender, age, BMI, smoking status, as well as dietary habits were entered in the model, with dioxin level as an outcome estimate. Both the logistic and linear model confirmed the contribution of dietary intake to the dioxin activity measured in serum. Also BMI and region were found to be associated with dioxin activity levels.     

Approaches to integrating indicators into 3D landscape visualisations and their benefits for participative planning situations

In discussing issues of landscape change, the complex relationships in the landscape have to be assessed. In participative planning processes, 3D visualisations have a high potential as an aid in understanding and communicating characteristics of landscape conditions by integrating visual and non-visual landscape information. Unclear is, which design and how much interactivity is required for an indicator visualisation that would suit stakeholders best in workshop situations. This paper describes the preparation and application of three different types of integrated 3D visualisations in workshops conducted in the Entlebuch UNESCO Biosphere Reserve (CH). The results reveal that simple representations of a complex issue created by draping thematic maps on the 3D model can make problematic developments visible at a glance; that diagrams linked to the spatial context can help draw attention to problematic relationships not considered beforehand; and that the size of species as indicators of conditions of the landscape's production and biotope function seems to provide a common language for stakeholders with different perspectives. Overall, the of the indicators the functions required to assist in information processing. Further research should focus on testing the effectiveness of the integrated visualisation tools in participative processes for the general public.     

Polymerase chain reaction analysis of the cystic fibrosis ΔF508 mutation in human blastomeres following oocyte injection of a single sperm from a carrier

The efficiency of the polymerase chain reaction (PCR) in detecting the cystic fibrosis (CF) ΔF508 mutation (which is the most common mutation of CF) was assessed in single human blastomeres. Twenty-one human immature oocytes (germinal-vesicle-stage oocytes) that had been donated for research were matured in vitro and a single spermatozoon from a carrier of the CF ΔF508 mutation was injected into the ooplasm. Fourteen embryos were obtained after intracytoplasmic sperm injection (ICSI). PCR analysis was carried out on 70 single blastomeres isolated from these 14 embryos. The results showed that the efficiency of DNA amplification by PCR in single nucleate blastomeres was 94 per cent (59/63). There were no false-positive results since none of the blank samples or the blastomeres without a nucleus showed an amplified signal. We found that nine embryos were homozygous for the unaffected genotype and that four embryos were heterozygous since they contained both the unaffected and the ΔF508 genotype. In a four-cell embryo, we observed the homozygous unaffected genotype in one blastomere and a heterozygous ΔF508/unaffected genotype in the other three blastomeres.     

Assessment of carbon leakage in multiple carbon-sink projects: a case study in Jambi Province,Indonesia

Rehabilitation of degraded forest land through implementation of carbon-sink projects can increase terrestrial carbon (C) stock. However, carbon emissions outside the project boundary, which is commonly referred to as leakage, may reduce or negate the sequestration benefits. This study assessed leakage from carbon-sink projects that could potentially be implemented in the study area comprised of 11 sub-districts in the Batanghari District, Jambi Province, Sumatra, Indonesia. The study estimates the probability of a given land use/cover being converted into other uses/cover, by applying a logit model. The predictor variables were: proximity to the center of the land use area, distance to transportation channel (road or river), area of agricultural land, unemployment (number of job seekers), job opportunities, population density and income. Leakage was estimated by analyzing with and without carbon-sink projects scenarios. Most of the predictors were estimated as being significant in their contribution to land use cover change. The results of the analysis show that leakage in the study area can be large enough to more than offset the project’s carbon sequestration benefits during the period 2002–2012. However, leakage results are very sensitive to changes of carbon density of the land uses in the study area. By reducing C-density of lowland and hill forest by about 10% for the baseline scenario, the leakage becomes positive. Further data collection and refinement is therefore required. Nevertheless, this study has demonstrated that regional analysis is a useful approach to assess leakage.